Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Product Name | NFE2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription factor NF-E2 45 kDa subunit, Leucine zipper protein NF-E2, Nuclear factor, erythroid-derived 2 45 kDa subunit, p45 NF-E2, NFE2 |
| Product description | Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.1) Wang, W., et al. Blood 116(2):254-266(2010) |
| Immunogen | This NFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-340 amino acids from the C-terminal region of human NFE2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFE2 |
|---|---|
| Alternative Names | Transcription factor NF-E2 45 kDa subunit Leucine zipper protein NF-E2 Nuclear factor erythroid-derived 2 45 kDa subunit p45 NF-E2 NFE2 |
| Molecular Weight(MW) | 41 kDa |
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
