WB
K-562 cells were subjected to SDS PAGE followed by western blot with NFE2 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer using NFE2 antibody at dilution of 1:50 (under 10x lens).ICC/IF
Immunofluorescent analysis of HepG2 cells, using NFE2 antibody at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).IP
IP result of anti-NFE2 (IP:4ug; Detection:1:500) with K-562 cells lysate 3440ug.| Product Name | NFE2 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human NFE2 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 0.45mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFE2 |
|---|---|
| Gene Synonyms | p45 NF-E2 |
| Gene Full Name | nuclear factor, erythroid 2 |
| Gene Summary | Enables WW domain binding activity; identical protein binding activity; and sequence-specific double-stranded DNA binding activity. Contributes to cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to act upstream of or within several processes, including labyrinthine layer blood vessel development; negative regulation of bone mineralization; and positive regulation of peptidyl-lysine acetylation. Part of RNA polymerase II transcription regulator complex and protein-DNA complex. [provided by Alliance of Genome Resources, May 2025] |
| Alternative Names | p45 NF E2 p45 Nuclear factor erythroid-derived 2 45 kDa subunit NF E2 Leucine zipper protein NF-E2 |
| Molecular Weight(MW) | 41kDa(Observed MW:45 kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
K-562 cells were subjected to SDS PAGE followed by western blot with NFE2 antibody at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human colon cancer using NFE2 antibody at dilution of 1:50 (under 10x lens).
ICC/IF
Immunofluorescent analysis of HepG2 cells, using NFE2 antibody at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP
IP result of anti-NFE2 (IP:4ug; Detection:1:500) with K-562 cells lysate 3440ug.| Application Notes | WB:1:500-1:1000 IHC:1:20-1:200 ICC/IF:1:20-1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
