Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Product Name | NFKBIA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFKBIA, IKBA, MAD-3, NFKBI |
| Product description | NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).1) Janssen, R., et al., (2004) J. Exp. Med. 200 (5), 559-568. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFKBIA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NFKBIA antibody can be used for detection of NFKBIA by ELISA at 1:1562500. NFKBIA antibody can be used for detection of NFKBIA by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIA |
|---|---|
| Alternative Names | NFKBIA IKBA MAD-3 NFKBI |
| Molecular Weight(MW) | 36 kDa |
Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10410 - Fetal Lung Tissue Lysate |
|---|---|
| Application Notes | NFKBIA antibody can be used for detection of NFKBIA by ELISA at 1:1562500. NFKBIA antibody can be used for detection of NFKBIA by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFKBIA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
