Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.Application
Antibody used in WB on Mouse, HeLa at: 1:500 (Lane 1: 100ug mouse liver lysate ,Lane 2: 100ug mouse brain lysate ,Lane 3: 100ug mouse heart lysate ,Lane 4: 100ug mouse kidney lysate ,Lane 5: 100ug mouse lung lysate ,Lane 6: 100ug mouse thymus lysate ,Lane 7: 100ug mouse spleen lysate ,Lane 8: 100ug mouse testis lysate ,Lane 9: 100ug HeLa cell lysate).| Product Name | NFKBIB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFKBIB, IKBB, TRIP9 |
| Product description | NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664 or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).1) Joo, M., et al., (2005) J. Virol. 79 (12), 7648-7657. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFKBIB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NFKBIB antibody can be used for detection of NFKBIB by ELISA at 1:2500. NFKBIB antibody can be used for detection of NFKBIB by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIB |
|---|---|
| Alternative Names | NFKBIB IKBB TRIP9 |
| Molecular Weight(MW) | 38 kDa |
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Application
Antibody used in WB on Mouse, HeLa at: 1:500 (Lane 1: 100ug mouse liver lysate ,Lane 2: 100ug mouse brain lysate ,Lane 3: 100ug mouse heart lysate ,Lane 4: 100ug mouse kidney lysate ,Lane 5: 100ug mouse lung lysate ,Lane 6: 100ug mouse thymus lysate ,Lane 7: 100ug mouse spleen lysate ,Lane 8: 100ug mouse testis lysate ,Lane 9: 100ug HeLa cell lysate).| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NFKBIB antibody can be used for detection of NFKBIB by ELISA at 1:2500. NFKBIB antibody can be used for detection of NFKBIB by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFKBIB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
