Application
Confocal immunofluorescent analysis of NFKBIL1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in CEM cell line lysates (35ug/lane).Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | NFKBIL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NF-kappa-B inhibitor-like protein 1, Inhibitor of kappa B-like protein, I-kappa-B-like protein, IkappaBL, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1, NFKBIL1, IKBL |
| Product description | This gene encodes a divergent member of the I-kappa-B family of proteins. Its function has not been determined. The gene lies within the major histocompatibility complex (MHC) class I region on chromosome 6. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].1) Clancy, R.M., et al. Arthritis Rheum. 62(11):3415-3424(2010) |
| Immunogen | This NFKBIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human NFKBIL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIL1 |
|---|---|
| Alternative Names | NF-kappa-B inhibitor-like protein 1 Inhibitor of kappa B-like protein I-kappa-B-like protein IkappaBL Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 NFKBIL1 IKBL |
| Molecular Weight(MW) | 43 kDa |
Application
Confocal immunofluorescent analysis of NFKBIL1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in CEM cell line lysates (35ug/lane).
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
