Application
Confocal immunofluorescent analysis of NFKBIL1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).Application
NFKBIL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | NFKBIL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NF-kappa-B inhibitor-like protein 1, Inhibitor of kappa B-like protein, I-kappa-B-like protein, IkappaBL, Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1, NFKBIL1, IKBL |
| Product description | This gene encodes a divergent member of the I-kappa-B family of proteins. Its function has not been determined. The gene lies within the major histocompatibility complex (MHC) class I region on chromosome 6. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].1) Clancy, R.M., et al. Arthritis Rheum. 62(11):3415-3424(2010) |
| Immunogen | This NFKBIL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 327-356 amino acids from the C-terminal region of human NFKBIL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFKBIL1 |
|---|---|
| Alternative Names | NF-kappa-B inhibitor-like protein 1 Inhibitor of kappa B-like protein I-kappa-B-like protein IkappaBL Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor-like 1 NFKBIL1 IKBL |
| Molecular Weight(MW) | 43 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Confocal immunofluorescent analysis of NFKBIL1 Antibody with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Application
NFKBIL1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
