NFKBIL2 is thought to be a negative regulator of NF-kappa-B mediated transcription. It may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus.The protein encoded by this gene is thought to be a negative regulator of NF-kappa-B mediated transcription. The encoded protein may bind NF-kappa-B complexes and trap them in the cytoplasm, preventing them from entering the nucleus and interacting with the DNA. Phosphorylation of this protein targets it for degradation by the ubiquitination pathway, which frees the NF-kappa-B complexes to enter the nucleus.1) Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFKBIL2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NFKBIL2 antibody can be used for detection of NFKBIL2 by ELISA at 1:62500. NFKBIL2 antibody can be used for detection of NFKBIL2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
NFKBIL2 antibody can be used for detection of NFKBIL2 by ELISA at 1:62500. NFKBIL2 antibody can be used for detection of NFKBIL2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFKBIL2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.