Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Product Name | NFX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFX1, DKFZp779G2416, MGC20369, NFX2, Tex42, TEG-42 |
| Product description | NFX1 binds to the X-box motif of MHC class II genes and represses their expression. NFX1 may play an important role in regulating the duration of an inflammatory response by limiting the period in which MHC class II molecules are induced by interferon-gamma. Isoform 3 binds to the X-box motif of TERT promoter and represses its expression. Together with PABPC1 or PABPC4, isoform 1 acts as a coactivator for TERT expression. NFX1 mediates E2-dependent ubiquitination.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.1) Katzenellenbogen, R.A., (2007) J. Virol. 81 (8), 3786-3796. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFX1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NFX1 antibody can be used for detection of NFX1 by ELISA at 1:62500. NFX1 antibody can be used for detection of NFX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFX1 |
|---|---|
| Alternative Names | NFX1 DKFZp779G2416 MGC20369 NFX2 Tex42 TEG-42 |
| Molecular Weight(MW) | 113 kDa |
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NFX1 antibody can be used for detection of NFX1 by ELISA at 1:62500. NFX1 antibody can be used for detection of NFX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
