Application
Antibody used in IHC on Human Testis.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Product Name | NFX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NFX1, DKFZp779G2416, MGC20369, NFX2, Tex42, TEG-42 |
| Product description | MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. NFX1 is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma.MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.1) Katzenellenbogen, R.A., (2007) J. Virol. 81 (8), 3786-3796. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NFX1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NFX1 antibody can be used for detection of NFX1 by ELISA at 1:312500. NFX1 antibody can be used for detection of NFX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFX1 |
|---|---|
| Alternative Names | NFX1 DKFZp779G2416 MGC20369 NFX2 Tex42 TEG-42 |
| Molecular Weight(MW) | 124 kDa |
Application
Antibody used in IHC on Human Testis.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NFX1 antibody can be used for detection of NFX1 by ELISA at 1:312500. NFX1 antibody can be used for detection of NFX1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NFX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
