Application
Immunohistochemistry of NIBRIN (CT) in human lung tissue with NIBRIN (CT) antibody at 2.5 μg/mL.Application
Immunofluorescence of NIBRIN in human lung tissue with NIBRIN antibody at 20 μg/mL.Application
Western blot analysis of NIBRIN in mouse lung tissue lysate with NIBRIN antibody at 1 μg/mL.| Product Name | NIBRIN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NIBRIN Antibody: ATV, NBS, P95, NBS1, AT-V1, AT-V2, Nibrin, Cell cycle regulatory protein p95 |
| Product description | NIBRIN Antibody: NIBRIN (NBN) is a member of the double-strand break repair complex MRE11/RAD50/NBN (MRN) which is involved in DNA double-strand break repair, DNA damage-induced checkpoint activation and plays a critical role in the maintenance of chromosome integrity. NIBRIN contains two modules found in cell cycle checkpoint proteins, a forkhead-associated domain adjacent to a breast cancer carboxy-terminal domain. Mutations in this gene are associated with Nijmegen breakage syndrome and maybe the cause of cancer predisposition and aplastic anemia.1) Carney JP, Maser RS, Olivares H, et al. The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response. Cell 1998; 93:477-86 |
| Immunogen | NIBRIN antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human NIBRIN. The immunogen is located within the last 50 amino acids of NIBRIN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NIBRIN antibody can be used for detection of NIBRIN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NBN |
|---|---|
| Alternative Names | NIBRIN Antibody: ATV NBS P95 NBS1 AT-V1 AT-V2 Nibrin Cell cycle regulatory protein p95 |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | Two alternatively spliced transcript isoforms of NIBRIN are known to exist. |
Application
Immunohistochemistry of NIBRIN (CT) in human lung tissue with NIBRIN (CT) antibody at 2.5 μg/mL.
Application
Immunofluorescence of NIBRIN in human lung tissue with NIBRIN antibody at 20 μg/mL.
Application
Western blot analysis of NIBRIN in mouse lung tissue lysate with NIBRIN antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | NIBRIN antibody can be used for detection of NIBRIN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NIBRIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NIBRIN Antibody is supplied in PBS containing 0.02% sodium azide. |
