Application
Western blot analysis of anti-HGK Antibody in jurkat cell line lysates (35ug/lane)| Product Name | NIK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEK kinase kinase 4, MEKKK 4, Nck-interacting kinase, MAP4K4, HGK, KIAA0687, NIK |
| Product description | HGK, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.1) Wright, J.H., et al., Mol. Cell. Biol. 23(6):2068-2082 (2003). Yao, Z., et al., J. Biol. Chem. 274(4):2118-2125 (1999). Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998). |
| Immunogen | This NIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-649 amino acids from the C-terminal region of human NIK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | MAP4K4 |
|---|---|
| Alternative Names | Mitogen-activated protein kinase kinase kinase kinase 4 HPK/GCK-like kinase HGK MAPK/ERK kinase kinase kinase 4 MEK kinase kinase 4 MEKKK 4 Nck-interacting kinase MAP4K4 HGK KIAA0687 NIK |
| Molecular Weight(MW) | 142 kDa |
Application
Western blot analysis of anti-HGK Antibody in jurkat cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
