Application
Immunofluorescence of NKX2-1 in human lung tissue with NKX2-1 antibody at 20 μg/mL.Application
Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 μg/mL.Application
Western blot analysis of NKX2-1 in rat lung tissue lysate with NKX2-1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | NKX2-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NKX2-1 Antibody: BCH, BHC, NK-2, TEBP, TTF1, NKX2A, TITF1, TTF-1, NKX2.1, Homeobox protein NK-2 homolog A |
| Product description | NKX2-1 Antibody: NKX2-1 (NK2 homeobox 1) has been identified as a thyroid-specific transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype and morphogenesis. It contains one homeobox DNA-binding domain. NKX2-1 also may play a role in lung development and surfactant homeostasis. Mutations and deletions in this gene are associated with benign hereditary chorea, congenital hypothyroidism, neonatal respiratory distress, and may be associated with thyroid cancer. NKX2-1 has been suggested to be a candidate suppressor of malignant progression.1) Lazzaro D, Price M, Felice MD, et al. The transcription factor TTF-1 is expressed at the onset of thyroid and lung morphogenesis and in restricted regions of the fetal brain. Development 1991; 113:1093-1104. |
| Immunogen | NKX2-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1. The immunogen is located within the first 50 amino acids of NKX2-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NKX2-1 antibody can be used for detection of NKX2-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NKX2-1 |
|---|---|
| Alternative Names | NKX2-1 Antibody: BCH BHC NK-2 TEBP TTF1 NKX2A TITF1 TTF-1 NKX2.1 Homeobox protein NK-2 homolog A |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | NKX2-1 antibody is predicted to not cross-react with other NK2 homeobox family members. At least three isoforms of NKX2-1 are known to exist; this antibody will detect all three. |
Application
Immunofluorescence of NKX2-1 in human lung tissue with NKX2-1 antibody at 20 μg/mL.
Application
Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 μg/mL.
Application
Western blot analysis of NKX2-1 in rat lung tissue lysate with NKX2-1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1462 - Rat Lung Tissue Lysate |
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| Application Notes | NKX2-1 antibody can be used for detection of NKX2-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NKX2-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NKX2-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
