Application
Immunofluorescence of NKX2-4 in A20 cells with NKX2-4 antibody at 20 μg/mL.Application
Immunocytochemistry of NKX2-4 in A20 cells with NKX2-4 antibody at 2.5 μg/mL.Application
Western blot analysis of NKX2-4 in A20 cell lysate with NKX2-4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | NKX2-4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NKX2-4 Antibody: NKX2D, NKX2.4, NKX2D, Homeobox protein NK-2 homolog D |
| Product description | NKX2-4 Antibody: NKX2-4 (NK2 homeobox 4) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-4 is closely related to NKX2-1 and NKX2-4 and is specifically localized to the ventral diencephalon. Recent experiments in Xenopus have suggested that the homeodomain protein XANF is necessary for the activation of NKX2-4, but does not directly regulate its expression.1) Wang CC, Brodnicki T, Copeland NG, et al. Conserved linkage of NK-2 homeobox gene pairs Nkx2-2/2-4 and Nkx2-1/2-9 in mammals. Mamm. Genome 2000; 11:466-8. |
| Immunogen | NKX2-4 antibody was raised against an 18 amino acid synthetic peptide near the center of human NKX2-4. The immunogen is located within amino acids 220 - 270 of NKX2-4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| NKX2-4 antibody can be used for detection of NKX2-4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NKX2-4 |
|---|---|
| Alternative Names | NKX2-4 Antibody: NKX2D NKX2.4 NKX2D Homeobox protein NK-2 homolog D |
| Sequence Similarities | 2) Cat. No. 17-208 - A-20 Cell Slide |
| Tissue Specificity | NKX2-4 antibody is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of NKX2-4 are known to exist; this antibody will detect both isoforms. |
Application
Immunofluorescence of NKX2-4 in A20 cells with NKX2-4 antibody at 20 μg/mL.
Application
Immunocytochemistry of NKX2-4 in A20 cells with NKX2-4 antibody at 2.5 μg/mL.
Application
Western blot analysis of NKX2-4 in A20 cell lysate with NKX2-4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | NKX2-4 antibody can be used for detection of NKX2-4 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NKX2-4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NKX2-4 Antibody is supplied in PBS containing 0.02% sodium azide. |
