Nkx2-4 belongs to the NK-2 homeobox family and contains 1 homeobox DNA-binding domain. In the embryo it is detected in the posterior hypotalamus and later in the head. In the adult it is detected only in testis. Nkx2-4 may be a transcription factor.1) Marin, O., et al., (2002) Development 129 (3), 761-773.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse NKX2-4.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NKX2-4 antibody can be used for detection of NKX2-4 by ELISA at 1:312500. NKX2-4 antibody can be used for detection of NKX2-4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Testis at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1313 - Human Testis Tissue Lysate
Application Notes
NKX2-4 antibody can be used for detection of NKX2-4 by ELISA at 1:312500. NKX2-4 antibody can be used for detection of NKX2-4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NKX2-4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.