Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in WB on Human CaCO3 at 1:10.| Product Name | NOX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOX1, GP91-2, MOX1, NOH-1, NOH1 |
| Product description | Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox.Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv. The NOH-1S currents were reversibly blocked by zinc, a known H+ channel inhibitor. The NOH-1S variant does not contain an electron transport chain and it is thought that H+ conductance is its main physiologic function, whereas NOH-1L may conduct H+ ions as part of its electron transport mechanism.1) Kuwano, Y., Am. J. Physiol., Cell Physiol. 290 (2), C433-C443 (2006). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NOX1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NOX1 antibody can be used for detection of NOX1 by ELISA at 1:62500. NOX1 antibody can be used for detection of NOX1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOX1 |
|---|---|
| Alternative Names | NOX1 GP91-2 MOX1 NOH-1 NOH1 |
| Molecular Weight(MW) | 65 kDa, 59 kDa |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in WB on Human CaCO3 at 1:10.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NOX1 antibody can be used for detection of NOX1 by ELISA at 1:62500. NOX1 antibody can be used for detection of NOX1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NOX1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
