WB
Western blot analysis of anti-NOX1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain whole cell lysates, Lane 2: mouse brain whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOX1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for NOX1 at approximately 65 kDa. The expected band size for NOX1 is at 65 kDa.IHC
IHC analysis of NOX1 using anti-NOX1 antibody. NOX1 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to NOX1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of rat NOX1, different from the related mouse sequence by one amino acid. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOX1 |
|---|---|
| Gene Synonyms | MOX1 NOH1 NOH-1 GP91-2 NOH-1L |
| Gene Full Name | NADPH oxidase 1 |
| Gene Summary | This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012] Enables NAD(P)H oxidase H2O2-forming activity and superoxide-generating NAD(P)H oxidase activity. Involved in several processes, including cellular response to mercury ion; cellular response to platelet-derived growth factor stimulus; and positive regulation of cellular component organization. Part of NADPH oxidase complex. Used to study Parkinson's disease; pulmonary hypertension; transient cerebral ischemia; and vascular dementia. Biomarker of middle cerebral artery infarction; pulmonary hypertension; and traumatic brain injury. Orthologous to human NOX1 (NADPH oxidase 1). [provided by Alliance of Genome Resources, Nov 2024] |
| Alternative Names | GP91 2 Mitogenic oxidase 1 MOX 1 MOX1 NADPH oxidase 1 NOH 1 NOH1 NOX 1 NOX1 |
| Molecular Weight(MW) | 65kDa |
| Cellular Localization | Cell membrane,Cell projection, invadopodium membrane |
WB
Western blot analysis of anti-NOX1 antibody. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain whole cell lysates, Lane 2: mouse brain whole cell lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-NOX1 antigen affinity purified polyclonal antibody and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for NOX1 at approximately 65 kDa. The expected band size for NOX1 is at 65 kDa.
IHC
IHC analysis of NOX1 using anti-NOX1 antibody. NOX1 was detected in a paraffin-embedded section of rat colon tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
