Application
Immunohistochemistry of NOX1 in human kidney tissue with NOX1 antibody at 5 μg/ml.Application
Immunofluorescence of NOX1 in human kidney tissue with NOX1 antibody at 20 μg/ml.Application
Western blot analysis of NOX1 in 293 cell lysate with NOX1 antibody at 1 μg/ml.| Product Name | NOX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOX1 Antibody: MOX1, NOH1, NOH-1, GP91-2, MOX1, NADPH oxidase 1, Mitogenic oxidase 1, NOX-1 |
| Product description | Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress (1). NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox (1). Three splice variants of NOX1 have been identified, NOH-1L, NOH-1S and NOH-1Lv (2). NOH-1S is a voltage-gated proton channel that participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain (1-3). NOX1 have the potential to be effective treatments for a range of ischemic diseases (4).1) Helmcke I, Heumuller S, Tikkanen R, et al. Identification of structural elements in Nox1 and Nox4 controlling localization and activity. Antioxid. Redox Signal. 2009; 11:1279-87. |
| Immunogen | NOX1 antibody was raised against a 15 amino acid peptide near the center of human NOX1. The immunogen is located within amino acids 210 - 260 of NOX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NOX1 antibody can be used for detection of NOX1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOX1 |
|---|---|
| Alternative Names | NOX1 Antibody: MOX1 NOH1 NOH-1 GP91-2 MOX1 NADPH oxidase 1 Mitogenic oxidase 1 NOX-1 |
| Molecular Weight(MW) | Predicted: 62 kDa Observed: 64 kDa |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | NOX1 antibody is human specific. |
Application
Immunohistochemistry of NOX1 in human kidney tissue with NOX1 antibody at 5 μg/ml.
Application
Immunofluorescence of NOX1 in human kidney tissue with NOX1 antibody at 20 μg/ml.
Application
Western blot analysis of NOX1 in 293 cell lysate with NOX1 antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | NOX1 antibody can be used for detection of NOX1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NOX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | NOX1 antibody is supplied in PBS containing 0.02% sodium azide. |
