Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.Application
Antibody used in IHC on Human Colon at 5.0 ug/ml.| Product Name | NR1I3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR1I3, CAR, CAR1, MB67, MGC150433, MGC97144, MGC97209 |
| Product description | NR1I3 mediates the induction of transcription of cytochrome P450 (CYP) genes by phenobarbital (PB) and PB-type inducers. NR1I3 activation induces hepatic expression of detoxification enzymes and transporters and increases liver size. NR1I3 can also regulate both liver homeostasis and tumorigenesis in response to xenobiotic stresses. This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene.1) Osabe, M., (2008) Biochem. Biophys. Res. Commun. 369 (4), 1027-1033. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR1I3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NR1I3 antibody can be used for detection of NR1I3 by ELISA at 1:312500. NR1I3 antibody can be used for detection of NR1I3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR1I3 |
|---|---|
| Alternative Names | NR1I3 CAR CAR1 MB67 MGC150433 MGC97144 MGC97209 |
| Molecular Weight(MW) | 35 kDa |
| Entrez Gene | 9970 |
|---|---|
| Protein Accession | NP_001070939 |
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Colon at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | NR1I3 antibody can be used for detection of NR1I3 by ELISA at 1:312500. NR1I3 antibody can be used for detection of NR1I3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR1I3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
