Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Skin at 5.0 ug/ml.| Product Name | NR2C1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR2C1, TR2, TR2-11 |
| Product description | The nuclear orphan receptors NR2C1 represses transcription and binds DNA as a homodimer. NR2C1 binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. NR2C1 may function as a negative modulator to suppress androgen receptor function in prostate cancer. NR2C1 may exert an important repressor in regulating ER activity in mammary glands. The nuclear orphan receptors TR2 (NR2C1) and TR4 form a heterodimer that binds to the epsilon and gamma globin promoter DR1 sites This gene encodes a nuclear hormone receptor characterized by a highly conserved DNA binding domain (DBD), a variable hinge region, and a carboxy-terminal ligand binding domain (LBD) that is typical for all members of the steroid/thyroid hormone receptor superfamily. This protein also belongs to a large family of ligand-inducible transcription factors that regulate gene expression by binding to specific DNA sequences within promoters of target genes. Multiple alternatively spliced transcript variants have been described, but the full-length nature of some of these variants has not been determined.1) Lin, Y.L., (2006) Biochem. Biophys. Res. Commun. 350 (2), 430-436. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR2C1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NR2C1 antibody can be used for detection of NR2C1 by ELISA at 1:312500. NR2C1 antibody can be used for detection of NR2C1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR2C1 |
|---|---|
| Alternative Names | NR2C1 TR2 TR2-11 |
| Molecular Weight(MW) | 51 kDa |
| Entrez Gene | 7181 |
|---|---|
| Protein Accession | NP_001027458 |
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Skin at 5.0 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | NR2C1 antibody can be used for detection of NR2C1 by ELISA at 1:312500. NR2C1 antibody can be used for detection of NR2C1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR2C1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
