Application
Antibody used in WB on Human HCT15 at 2.5 ug/ml.| Product Name | NR2C1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR2C1, TR2 |
| Product description | The nuclear orphan receptors NR2C1 represses transcription and binds DNA as a homodimer. NR2C1 binds the IR7 element in the promoter of its own gene in an autoregulatory negative feedback mechanism. NR2C1 may function as a negative modulator to suppress androgen receptor function in prostate cancer. NR2C1 may exert an important repressor in regulating ER activity in mammary glands. The nuclear orphan receptors TR2 (NR2C1) and TR4 form a heterodimer that binds to the epsilon and gamma globin promoter DR1 sites1) Li, G., et al., (2003) Biochem. Biophys. Res. Commun. 310 (2), 384-390. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR2C1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NR2C1 antibody can be used for detection of NR2C1 by ELISA at 1:312500. NR2C1 antibody can be used for detection of NR2C1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NR2C1 |
|---|---|
| Alternative Names | NR2C1 TR2 |
| Molecular Weight(MW) | 67 kDa |
Application
Antibody used in WB on Human HCT15 at 2.5 ug/ml.| Positive Control | 1) HCT15 Cell Lysate |
|---|---|
| Application Notes | NR2C1 antibody can be used for detection of NR2C1 by ELISA at 1:312500. NR2C1 antibody can be used for detection of NR2C1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR2C1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
