Application
Immunohistochemistry of NSA1 in rat liver tissue with NSA1 antibody at 5 μg/mL.Application
Immunofluorescence of NSA1 in rat liver tissue with NSA1 antibody at 20 μg/mL.Application
Western blot analysis of NSA1 in mouse liver tissue lysate with NSA1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | NSA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NSA1 Antibody: NSA1, WD repeat-containing protein 74, NOP seven-associated protein 1 |
| Product description | NSA1 Antibody: The yeast nucleolar protein NOP7 is necessary for the maturation of 66S preribosomes and interacts with numerous other proteins. One such protein is an essential, conserved WD repeat protein, NOP seven-associated protein 1 (NSA1), that is also required for the yeast 66S ribosome assembly. NSA1 is also associated with the AAA ATPase Rix7, and release of NSA1 from a novel late nucleolar pre-60S requires the Rix7 function. NSA1 has also been found upregulated in mammalian cancer cells, suggesting it may also play a role in cell proliferation.1) Miles TD, Jakovljevic J, Horsey EW, et al. Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol. Cell Biol.2005; 25:10419-32. |
| Immunogen | NSA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NSA1. The immunogen is located within amino acids 320 - 370 of NSA1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| NSA1 antibody can be used for detection of NSA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | WDR74 |
|---|---|
| Alternative Names | NSA1 Antibody: NSA1 WD repeat-containing protein 74 NOP seven-associated protein 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
Application
Immunohistochemistry of NSA1 in rat liver tissue with NSA1 antibody at 5 μg/mL.
Application
Immunofluorescence of NSA1 in rat liver tissue with NSA1 antibody at 20 μg/mL.
Application
Western blot analysis of NSA1 in mouse liver tissue lysate with NSA1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | NSA1 antibody can be used for detection of NSA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | NSA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | NSA1 Antibody is supplied in PBS containing 0.02% sodium azide. |
