WB
Various lysates were subjected to SDS PAGE followed by western blot withWDR74 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WDR74 antibody at dilution of 1:1000 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).| Product Name | WDR74 Recombinant monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human WDR74 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:4000 ICC/IF:1:500-1:2000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | WDR74 |
|---|---|
| Gene Synonyms | Nsa1 |
| Gene Full Name | WD repeat domain 74 |
| Gene Summary | Involved in rRNA processing and ribosomal large subunit biogenesis. Located in nuclear exosome (RNase complex); nucleolus; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | NSA1 WD repeat domain 74 WD repeat-containing protein 74 |
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Nucleus. |
WB
Various lysates were subjected to SDS PAGE followed by western blot withWDR74 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WDR74 antibody at dilution of 1:1000 and Omnimabs® 488 Goat Anti-Rabbit IgG(H&L), CL594-Phalloidin (red).| Application Notes | WB:1:1000-1:4000 ICC/IF:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
