Application
Antibody used in IHC on Human kidney at 4.0-8.0 ug/ml.Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in WB on Human HeLa at: 1:300 (Lane 1: 7ug HeLa lysate+EGFP SiRNA , Lane 2: 7ug HeLa lysate+NXF1 SiRNA).| Product Name | NXF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NXF1, TAP, MEX67 |
| Product description | NXF1 is one member of a family of nuclear RNA export factor. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. NXF1 shuttles between the nucleus and the cytoplasm and binds in vivo to poly (A)+ RNA. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly (A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.1) Chen, I.H., (2005) J. Virol. 79 (7), 3949-3961. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NXF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| NXF1 antibody can be used for detection of NXF1 by ELISA at 1:312500. NXF1 antibody can be used for detection of NXF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NXF1 |
|---|---|
| Alternative Names | NXF1 TAP MEX67 |
| Molecular Weight(MW) | 68 kDa, 40 kDa |
Application
Antibody used in IHC on Human kidney at 4.0-8.0 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in WB on Human HeLa at: 1:300 (Lane 1: 7ug HeLa lysate+EGFP SiRNA , Lane 2: 7ug HeLa lysate+NXF1 SiRNA).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NXF1 antibody can be used for detection of NXF1 by ELISA at 1:312500. NXF1 antibody can be used for detection of NXF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NXF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
