Immunoblot
Lane 1: 7ug HeLa lysate+EGFP SiRNA Lane 2: 7ug HeLa lysate+NXF1 SiRNAPrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: NXF1Submitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto UniversityWestern blot
1.25ug/mlPositive Control: HepG2 cell lysate| Product Name | Rabbit anti-NXF1 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAP, MEX67 |
| Immunogen | The immunogen for anti-NXF1 antibody: synthetic peptide directed towards the N terminal of human NXF1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NXF1 |
|---|---|
| Gene Synonyms | TAP MEX67 |
| Gene Full Name | Nuclear RNA export factor 1 |
| Gene Summary | NXF1 is one member of a family of nuclear RNA export factor. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. NXF1 shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. |
| Alternative Names | TAP MEX67 |
| Molecular Weight(MW) | 68kDa |
| Sequence | 619 amino acids |
Immunoblot
Lane 1: 7ug HeLa lysate+EGFP SiRNA Lane 2: 7ug HeLa lysate+NXF1 SiRNAPrimary Antibody Dilution: 1:300Secondary Antibody: Anti-rabbit-HRPSecondary Antibody Dilution: 1:500Gene Name: NXF1Submitted by: Seiji Masuda, Kitashirakawa Oiwakecho, Kyoto University
Western blot
1.25ug/mlPositive Control: HepG2 cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
