OLIG2 is a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. OLIG2 is an essential regulator of ventral neuroectodermal progenitor cell fate. It is associated with T-cell acute lymphoblastic leukemia due to a chromosomal translocation t (14;21) (q11.2;q22). OLIG2 might play a role in learning deficits associated with Down syndrome.1) Sun, T., et al., (2006) Dev. Biol. 292 (1), 152-164.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human OLIG2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
OLIG2 antibody can be used for detection of OLIG2 by ELISA at 1:312500. OLIG2 antibody can be used for detection of OLIG2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Fetal Lung at 1 ug/ml.
Application
Antibody used in IHC on Human at 1:500.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
OLIG2 antibody can be used for detection of OLIG2 by ELISA at 1:312500. OLIG2 antibody can be used for detection of OLIG2 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store OLIG2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.