Application
Immunohistochemistry of OLIG2 in rat brain tissue with OLIG2 antibody at 5 μg/mL.Application
Immunofluorescence of OLIG2 in mouse brain tissue with OLIG2 Antibodyat 20 μg/mL.Application
Immunohistochemistry of OLIG2 in mouse brain tissue with OLIG2 Antibodyat 5 μg/mL.Application
Western blot analysis of OLIG2 in EL4 cell lysate with OLIG2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of OLIG2 in rat brain tissue with OLIG2 antibody at 20 μg/mL.| Product Name | OLIG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OLIG2 Antibody: BHLHB1, OLIGO2, RACK17, PRKCBP2, bHLHe19, BHLHB1, BHLHE19, Oligodendrocyte transcription factor 2, Class B basic helix-loop-helix protein 1, Oligo2 |
| Product description | The oligodendrocyte transcription factors 1 and 2 (OLIG1 and OLIG2, respectively) make up part of basic helix-loop-helix (bHLH) family of transcription factors that are specifically expressed in zones of the neuroepithelium from which oligodendrocyte precursors emerge (1). Both OLIG1 and OLIG2 genes are downstream targets of Sonic hedgehog and are expressed exclusively in the central nervous system (2). OLIG2 is first observed in the ventral most p3 progenitor domain of the ventral neural tube while OLIG1 is first expressed in the dorsal portion of the p3 domain (2). Mice overexpressing OLIG2 exhibit impaired potassium channel expression in neural progenitors and proliferation of these cells similar to that seen in Down Syndrome, suggesting that OLIG2 may play a role in this pathology (3).1) Zhou Q, Wang S, and Anderson DJ. Identification of a novel family of oligodendrocyte lineage-specific basic helix-loop-helix transcription factors. Neuron 2000; 25:331-43. |
| Immunogen | OLIG2 antibody was raised against a 15 amino acid peptide near the amino terminus of human OLIG2. The immunogen is located within amino acids 80 - 130 of OLIG2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| OLIG2 antibody can be used for detection of OLIG2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OLIG2 |
|---|---|
| Alternative Names | OLIG2 Antibody: BHLHB1 OLIGO2 RACK17 PRKCBP2 bHLHe19 BHLHB1 BHLHE19 Oligodendrocyte transcription factor 2 Class B basic helix-loop-helix protein 1 Oligo2 |
| Molecular Weight(MW) | Predicted: 36 kDa Observed: 37 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (100%) |
| Tissue Specificity | OLIG2 antibody is human, mouse and rat reactive. It is predicted to not cross-react with other members of the OLIG family of proteins. At least three isoforms of OLIG2 are known to exist; this antibody will detect all three isoforms. |
Application
Immunohistochemistry of OLIG2 in rat brain tissue with OLIG2 antibody at 5 μg/mL.
Application
Immunofluorescence of OLIG2 in mouse brain tissue with OLIG2 Antibodyat 20 μg/mL.
Application
Immunohistochemistry of OLIG2 in mouse brain tissue with OLIG2 Antibodyat 5 μg/mL.
Application
Western blot analysis of OLIG2 in EL4 cell lysate with OLIG2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of OLIG2 in rat brain tissue with OLIG2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | OLIG2 antibody can be used for detection of OLIG2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | OLIG2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | OLIG2 Antibody is supplied in PBS containing 0.02% sodium azide. |
