Application
Flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue with OLIG3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of OLIG3 Antibody in mouse spleen tissue lysates (35ug/lane)| Product Name | OLIG3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Oligodendrocyte transcription factor 3, Oligo3, Class B basic helix-loop-helix protein 7, bHLHb7, Class E basic helix-loop-helix protein 20, bHLHe20, OLIG3, BHLHB7, BHLHE20 |
| Product description | OLIG3 may determine the distinct specification program of class A neurons in the dorsal part of the spinal cord and suppress specification of class B neurons.1) Muller,T., et.al., Genes Dev. 19 (6), 733-743 (2005) |
| Immunogen | This OLIG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-100 amino acids from the Central region of human OLIG3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OLIG3 |
|---|---|
| Alternative Names | Oligodendrocyte transcription factor 3 Oligo3 Class B basic helix-loop-helix protein 7 bHLHb7 Class E basic helix-loop-helix protein 20 bHLHe20 OLIG3 BHLHB7 BHLHE20 |
| Molecular Weight(MW) | 29 kDa |
Application
Flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue with OLIG3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of OLIG3 Antibody in mouse spleen tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
