WB
Western blot analysis of 293T cell lysate. Using OLIG3 monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.IHC
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG3 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.IHC
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG3 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.ICC/IF
4% Paraformaldehyde-fixed U-87MG (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (OLIG3) monoclonal Antibody, unconjugated 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.| Product Name | OLIG3 Recombinant Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthesized peptide derived from human Oligo3: 230-272. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-500 ICC/IF:1:50-200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | OLIG3 |
|---|---|
| Gene Synonyms | Bhlhb7 bHLHe20 |
| Gene Full Name | oligodendrocyte transcription factor 3 |
| Gene Summary | Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in axon development; positive regulation of transcription by RNA polymerase II; and sensory organ development. Predicted to act upstream of or within negative regulation of transcription by RNA polymerase II; spinal cord motor neuron cell fate specification; and spinal cord motor neuron migration. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | Bhlhb7 bHLHe20 oligo3 OLIG3_HUMAN OLIG3 Class B basic helix-loop-helix protein 7 (bHLHb7) Class E basic helix-loop-helix protein 20 (bHLHe20) OLIG3_MOUSE Oligodendrocyte-specific bHLH transcription factor 3 oligodendrocyte transcription factor 3 |
| Molecular Weight(MW) | 29kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of 293T cell lysate. Using OLIG3 monoclonal antibody at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG3 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
IHC
Paraformaldehyde-fixed, paraffin embedded Human Skin; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with OLIG3 Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the HRP and DAB staining.
ICC/IF
4% Paraformaldehyde-fixed U-87MG (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (OLIG3) monoclonal Antibody, unconjugated 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.| Application Notes | WB:1:500-1:2000 IHC:1:100-500 ICC/IF:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | pH7.4, 150mM NaCl, 0.02% Proclin300 and 50% Glycerol. |
