Application
To detect Human OPG by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human OPG (XP-5253Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human OPG.Application
To detect Human OPG by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human OPG (XP-5253Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human OPG.Application
To detect hOPG by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human OPG (XP-5253Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOPG.| Product Name | Osteoprotegerin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OPG, TR1, OCIF, OPG, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, |
| Product description | Osteoprotegerin (OPG) is a member of the TNF receptor superfamily. This protein is an osteoblast-secreted decoy receptor that functions as a negative regulator of bone resorption. This protein specifically binds to its ligand, osteoprotegerin ligand (TNFSF11/OPGL), both of which are key extracellular regulators of osteoclast development. Studies of the mouse counterpart also suggest this protein and its ligand play a role in lymph-node organogenesis and vascular calcification. Alternatively spliced transcript variants of the gene have been reported, but their full length nature has not been determined. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hOPG (human Osteoprotegrin). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: To detect hOPG by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hOPG. Sandwich: To detect hOPG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human OPG (XP-5253Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOPG. Western Blot: To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFRSF11B |
|---|---|
| Alternative Names | OPG TR1 OCIF OPG Tumor necrosis factor receptor superfamily member 11B Osteoclastogenesis inhibitory factor |
Application
To detect Human OPG by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human OPG (XP-5253Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human OPG.
Application
To detect Human OPG by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human OPG (XP-5253Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human OPG.
Application
To detect hOPG by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human OPG (XP-5253Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOPG.| Application Notes | ELISA: To detect hOPG by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hOPG. Sandwich: To detect hOPG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human OPG (XP-5253Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hOPG. Western Blot: To detect hOPG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOPG is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Osteoprotegerin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
