Application
Immunofluorescence analysis of methanol - fixed L - 02 cells using P11 monoclonal antiobdy showing cytoplasmic and membrane localization (top). Flow cytometic analysis of L - 02 cells using monoclonal antibody to human P11 (bottom).Application
Western blot analysis using P11 monoclonal antibody against truncated P11 recombinant protein| Product Name | P11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Placental protein 11, PP11, P11, PRSS26 |
| Product description | S100 calcium binding protein A10 (S100A10/P11), The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.1) Svenningsson P, Greengard P. Curr Opin Pharmacol. 2007;7(1):27-32. |
| Immunogen | Ni-NTA purified truncated recombinant P11 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSWB |
| Dilution ELISA:Propose dilution 1:10,000 WB:1:100 - 1:500 IF:1:100 - 1:500 FCM:1:100 - 1:500 Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ENDOU |
|---|---|
| Alternative Names | Placental protein 11 PP11 P11 PRSS26 |
Application
Immunofluorescence analysis of methanol - fixed L - 02 cells using P11 monoclonal antiobdy showing cytoplasmic and membrane localization (top). Flow cytometic analysis of L - 02 cells using monoclonal antibody to human P11 (bottom).
Application
Western blot analysis using P11 monoclonal antibody against truncated P11 recombinant protein| Application Notes | Dilution ELISA:Propose dilution 1:10,000 WB:1:100 - 1:500 IF:1:100 - 1:500 FCM:1:100 - 1:500 Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | P11 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
