Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Ab-424). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p70 S6 Kinase (Ab-424).Application
Western blot analysis of lysed extracts from 293 cells using p70 S6 Kinase (Ab-424).| Product Name | p70 S6 Kinase Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | S6K, PS6K, S6K1, STK14A, p70-S6K, p70S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha, PKR, PRKR, KS6B1, P70-S6K, RPS6KB1, S6K |
| Product description | RPS6KB1 phosphorylates the Ribosomal Protein-S6. Activation of RPS6KB1 requires a complex, ordered series of conformational changes and phosphorylation reactions. While the role of sequential, multi-site phosphorylation has been extensively detailed, characterization of the priming step required to initiate this cascade has remained elusive. Probably this priming process is dependent on calcium. Calcium-dependent regulation of RPS6KB1 does not specifically target Thr-229 and Thr-389, the key regulatory phosphorylation sites; rather, calcium chelation results in a global inhibition of RPS6KB1 phosphorylation. The initial calcium-dependent process is required to release an inhibitory interaction between the C- and N-termini of RPS6KB1, thus allowing phosphorylation of key domains. The priming event involves formation of a calcium-dependent protein complex that releases the interaction between the N- and C-termini. RPS6KB1 is then accessible for activation by the kinases that target the known regulatory phosphorylation sites .1) Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273 (24): 15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X. F, et al. (2003) Oncogene 22: 484–97 |
| Immunogen | p70 S6 Kinase (Ab-424) antibody was raised against a peptide sequence around aa.422~426 (P-V-S-P-V) derived from Human p70S6k. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KB1 |
|---|---|
| Alternative Names | S6K PS6K S6K1 STK14A p70-S6K p70S6KA p70-alpha S6K-beta-1 p70(S6K)-alpha PKR PRKR KS6B1 P70-S6K RPS6KB1 S6K |
| Molecular Weight(MW) | 70, 85 kDa |
| Tissue Specificity | This antibody detects endogenous level of total p70 S6 Kinase protein. |
| Entrez Gene | 6198 |
|---|---|
| Protein Accession | NP_003152.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Ab-424). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p70 S6 Kinase (Ab-424).
Application
Western blot analysis of lysed extracts from 293 cells using p70 S6 Kinase (Ab-424).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
