Application
Western blot analysis of extracts from Jurkat cells treated with Insulin using Phospho-p70 S6 Kinase (Thr389/412) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | p70 S6 Kinase (phospho Thr389/412) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KS6B1, P70-S6K, RPS6KB1, Ribosomal protein S6 kinase, polypeptide 1 |
| Product description | Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.1) Hong S, et al. (2014) J Biol Chem |
| Immunogen | p70 S6 Kinase (phospho Thr389/412) antibody was raised against a peptide sequence around phosphorylation site of threonine389/412 (G-F-T (p)-Y-V) derived from Human p70 S6 Kinase . |
| Modification | p-Thr389/412 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KB1 |
|---|---|
| Alternative Names | KS6B1 P70-S6K RPS6KB1 Ribosomal protein S6 kinase polypeptide 1 |
| Molecular Weight(MW) | 70 kDa |
| Tissue Specificity | The antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at tyrosine 389/tyrosine 412. |
| Entrez Gene | 6198 |
|---|---|
| Protein Accession | NP_001258971.1 |
Application
Western blot analysis of extracts from Jurkat cells treated with Insulin using Phospho-p70 S6 Kinase (Thr389/412) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
