Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Ser424). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, centrosomal and nuclear staining using p70 S6 Kinase (Phospho-Ser424).Application
Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase (Phospho-Ser424).| Product Name | p70 S6 Kinase (phospho Ser424) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | S6K, PS6K, S6K1, STK14A, p70-S6K, p70S6KA, p70-alpha, S6K-beta-1, p70(S6K)-alpha, HCP, PTP1C, KS6B1, P70-S6K, RPS6KB1, S6K |
| Product description | Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation.1) Satoru Eguchi et al. (1999) J Biol Chem, Vol. 274: 36843-36851Papst PJ, et al. (1998) J Biol Chem. 273 (24): 15077-84. Ulrike Krause et al. (2002) Eur. J. Biochem. 269: 3751-3759 cLe, X. F, et al. (2003) Oncogene 22: 484–97 |
| Immunogen | p70 S6 Kinase (Phospho-Ser424) antibody was raised against a peptide sequence around phosphorylation site of serine 424 (P-V-S (p) -P-V) derived from Human p70 S6 Kinase. |
| Modification | p-Ser424 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RPS6KB1 |
|---|---|
| Alternative Names | S6K PS6K S6K1 STK14A p70-S6K p70S6KA p70-alpha S6K-beta-1 p70(S6K)-alpha HCP PTP1C KS6B1 P70-S6K RPS6KB1 S6K |
| Molecular Weight(MW) | 70, 85 kDa |
| Tissue Specificity | This antibody detects endogenous level of p70 S6 Kinase only when phosphorylated at serine 424. |
| Entrez Gene | 6198 |
|---|---|
| Protein Accession | NP_003152.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p70 S6 Kinase (Phospho-Ser424). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic, centrosomal and nuclear staining using p70 S6 Kinase (Phospho-Ser424).
Application
Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using p70 S6 Kinase (Phospho-Ser424).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
