Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in IHC on Human brain stems (NT2) at 1:500.| Product Name | PABPC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PABPC1, PAB1, PABP, PABP1, PABPC2, PABPL1 |
| Product description | The poly (A)-binding protein (PABP), which is found complexed to the 3-prime poly (A) tail of eukaryotic mRNA, is required for poly (A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.The poly (A)-binding protein (PABP), which is found complexed to the 3-prime poly (A) tail of eukaryotic mRNA, is required for poly (A) shortening and translation initiation. In humans, the PABPs comprise a small nuclear isoform and a conserved gene family that displays at least 3 functional proteins: PABP1 (PABPC1), inducible PABP (iPABP, or PABPC4; MIM 603407), and PABP3 (PABPC3; MIM 604680). In addition, there are at least 4 pseudogenes, PABPCP1 to PABPCP4.1) Rivera, C.I. (2008) Virology 375 (1), 59-72. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PABPC1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PABPC1 antibody can be used for detection of PABPC1 by ELISA at 1:1562500. PABPC1 antibody can be used for detection of PABPC1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PABPC1 |
|---|---|
| Alternative Names | PABPC1 PAB1 PABP PABP1 PABPC2 PABPL1 |
| Molecular Weight(MW) | 71 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human brain stems (NT2) at 1:500.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PABPC1 antibody can be used for detection of PABPC1 by ELISA at 1:1562500. PABPC1 antibody can be used for detection of PABPC1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PABPC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
