Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PABPC1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PABPC1 Antibody in NIH-3T3 cell line lysates (35ug/lane)| Product Name | PABPC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Polyadenylate-binding protein 1, PABP-1, Poly(A)-binding protein 1, PABPC1, PAB1, PABP1, PABPC2 |
| Product description | PABPC1 binds the poly(A) tail of mRNA. It may be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. PABPC1 can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. It may be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.1) Le Clerc,S., et.al., J. Infect. Dis. 200 (8), 1194-1201 (2009) |
| Immunogen | This PABPC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human PABPC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PABPC1 |
|---|---|
| Alternative Names | Polyadenylate-binding protein 1 PABP-1 Poly(A)-binding protein 1 PABPC1 PAB1 PABP1 PABPC2 |
| Molecular Weight(MW) | 71 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PABPC1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PABPC1 Antibody in NIH-3T3 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
