Application
Immunofluorescence of PACS2 in mouse brain tissue with PACS2 antibody at 10 μg/ml.Application
Immunohistochemistry of PACS2 in mouse brain tissue with PACS2 antibody at 20 μg/mL.Application
Western blot analysis of PACS2 in mouse brain tissue lysate with PACS2 antibody at 1 μg/ml.WB
E, Evaluation of the expression level of PACS-2, the major tethering protein for MAMs.| Product Name | PACS2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PACS2 (phosphofurin acidic cluster sorting protein-2), PACS1L, is an 889 amino acid protein that localizes to both the mitochondrion and the lumen of the endoplasmic reticulum (ER) and belongs to the PACS family (1,2). It is expressed in a broad range of tissues with highest expression in skeletal muscle, brain, heart, testis and pancreas (2,). PACS2 interacts with Polycystin-2 and BID and functions as a sorting protein that regulates mitochondria-ER communication and is thought to be involved in ion channel trafficking, specifically direct cluster-containing ion channels to distinct subcellular compartments (3-5).1) Kottgen M, Benzing T, Simmen T, et al. Trafficking of TRPP2 by PACS proteins represents a novel mechanism of ion channel regulation. EMBO J. 2005; 24:705-16. |
| Immunogen | PACS2 antibody was raised against a 17 amino acid peptide near the center of human PACS2. The immunogen is located within amino acids 330 - 380 of PACS2. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IHC-P:1:50~1:200 IF:1:100~1:500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PACS2 |
|---|---|
| Alternative Names | Phosphofurin acidic cluster sorting protein 2 PACS-2 PACS1-like protein PACS1L |
| Molecular Weight(MW) | Predicted: 98 kDa Observed: 95 kDa |
| Tissue Specificity | PACS2 antibody is human, mouse and rat reactive. At least three isoforms of PACS2 are known to exist; this antibody will detect all three. PACS2 antibody is predicted to not cross-react with PACS1. |
Application
Immunofluorescence of PACS2 in mouse brain tissue with PACS2 antibody at 10 μg/ml.
Application
Immunohistochemistry of PACS2 in mouse brain tissue with PACS2 antibody at 20 μg/mL.
Application
Western blot analysis of PACS2 in mouse brain tissue lysate with PACS2 antibody at 1 μg/ml.
WB
E, Evaluation of the expression level of PACS-2, the major tethering protein for MAMs.| Positive Control | Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IHC-P:1:50~1:200 IF:1:100~1:500: |
| Form | Liquid |
|---|---|
| Storage Instructions | PACS2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | PACS2 antibody is supplied in PBS containing 0.02% sodium azide. |
