Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using PAK1 (Ab-212).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (Ab-212). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PAK1 (Ab-212).| Product Name | PAK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAKalpha, GLUH1, GLUR1, p21-activated kinase 1 |
| Product description | The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.1) Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819Thiel DA, et al. (2002) Curr Biol; 12:1227-1232Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052. |
| Immunogen | PAK1 (Ab-212) antibody was raised against a peptide sequence around aa. 210~214 (P-V-T-P-T) derived from Human PAK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK1 |
|---|---|
| Alternative Names | PAKalpha GLUH1 GLUR1 p21-activated kinase 1 |
| Molecular Weight(MW) | 68 kDa |
| Tissue Specificity | This antibody detects endogenous level of total PAK1protein. |
| Entrez Gene | 5058 |
|---|---|
| Protein Accession | NP_001122092.1 |
Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using PAK1 (Ab-212).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (Ab-212). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PAK1 (Ab-212).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
