Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (Phospho-Thr212). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PAK1 (Phospho-Thr212).Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with forskolin using PAK1 (Phospho-Thr212).| Product Name | PAK1 (phospho Thr212) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAKalpha, C1orf215, LAP18, OP18, p21-activated kinase 1, PAK-1, p65-PAK, Alpha-PAK |
| Product description | The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.1) Alexander K, et al. (2004) Mol Cell Biol; 24: 2808-2819Thiel DA, et al. (2002) Curr Biol; 12:1227-1232Rashid T, et al. (2001) J. Biol. Chem; 276: 49043 - 49052. |
| Immunogen | PAK1 (Phospho-Thr212) antibody was raised against a peptide sequence around phosphorylation site of threonine 212 (P-V-T (p) -P-T) derived from Human PAK1. |
| Modification | p-Thr212 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK1 |
|---|---|
| Alternative Names | PAKalpha C1orf215 LAP18 OP18 p21-activated kinase 1 PAK-1 p65-PAK Alpha-PAK |
| Molecular Weight(MW) | 68 kDa |
| Tissue Specificity | This antibody detects endogenous level of PAK1 only when phosphorylated at threonine 212. |
| Entrez Gene | 5058 |
|---|---|
| Protein Accession | NP_001122092.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PAK1 (Phospho-Thr212). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PAK1 (Phospho-Thr212).
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with forskolin using PAK1 (Phospho-Thr212).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
