Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 Antibody (T423) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Confocal immunofluorescent analysis of PAK1 Antibody (T423) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Application
Western blot analysis of PAK1 using rabbit polyclonal PAK1 Antibody (T423).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2).| Product Name | PAK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase PAK 1, Alpha-PAK, p21-activated kinase 1, PAK-1, p65-PAK, PAK1 |
| Product description | PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.1) Chen, S., et al., J. Biol. Chem. 278(22):20029-20036 (2003). |
| Immunogen | This PAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 401-430 amino acids from human PAK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK1 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase PAK 1 Alpha-PAK p21-activated kinase 1 PAK-1 p65-PAK PAK1 |
| Molecular Weight(MW) | 61 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 Antibody (T423) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Confocal immunofluorescent analysis of PAK1 Antibody (T423) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application
Western blot analysis of PAK1 using rabbit polyclonal PAK1 Antibody (T423).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
