Application
Immunocytochemistry of PARL in 3T3 cells with PARL antibody at 2.5 μg/mL.Application
Immunofluorescence of PARL in 3T3 cells with PARL antibody at 2.50 μg/mL.Application
Western blot analysis of PARL in 3T3 cell lysate with PARL antibody at (A) 1 and (B) 2 μg/mL.| Product Name | PARL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PARL Antibody: PSARL, PSARL1, RHBDS1, PRO2207, PSENIP2, PSARL, Presenilins-associated rhomboid-like protein, mitochondrial, Mitochondrial intramembrane cleaving protease PARL, Pbeta |
| Product description | PARL Antibody: PARL is a mitochondrial integral membrane protein and a member of the highly conserved rhomboid superfamily of membrane proteins. PARL is required for the control of apoptosis during postnatal growth and is required for the processing of OPA1, a protein that prevents the release of cytochrome C from the mitochondria in response to apoptotic signals. In lymphocytes and neurons, PARL's association with other proteins, such as the Bcl-2 family-related protein Hax1 and Omi can lead to the proteolytic processing of Omi by PARL, preventing the accumulation of Bax, thereby suppressing apoptosis. Variations in this gene have been associated with increased risk for type 2 diabetes. PARL may also play a role in the progression of Alzheimer's disease through its associations with presenilin-1 and -2. Multiple isoforms of PARL are known to exist.1) Pellegrini L, Passer BJ, Canelles M, et al. PAMP and PARL, two novel putative metalloproteases interacting with the COOH-terminus of Presenilin-1 and -2. J. Alzheimers Dis.2001; 3:181-90. |
| Immunogen | PARL antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human PARL. The immunogen is located within amino acids 40 - 90 of PARL. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PARL antibody can be used for detection of PARL by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PARL |
|---|---|
| Alternative Names | PARL Antibody: PSARL PSARL1 RHBDS1 PRO2207 PSENIP2 PSARL Presenilins-associated rhomboid-like protein mitochondrial Mitochondrial intramembrane cleaving protease PARL Pbeta |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
Application
Immunocytochemistry of PARL in 3T3 cells with PARL antibody at 2.5 μg/mL.
Application
Immunofluorescence of PARL in 3T3 cells with PARL antibody at 2.50 μg/mL.
Application
Western blot analysis of PARL in 3T3 cell lysate with PARL antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | PARL antibody can be used for detection of PARL by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PARL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PARL Antibody is supplied in PBS containing 0.02% sodium azide. |
