Application
Western blot analysis in A431 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human skin reacted with PAX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of K562 cells using PAX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PAX3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PAX3 probable is a transcription factor associated with development of alveolar rhabdomyosarcoma.1) Boutet,S.C., et.al., Cell 130 (2), 349-362 (2007) |
| Immunogen | This PAX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-126 amino acids from the N-terminal region of human PAX3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAX3 |
|---|---|
| Alternative Names | Paired box protein Pax-3 HuP2 PAX3 HUP2 |
| Molecular Weight(MW) | 53 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in A431 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human skin reacted with PAX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of K562 cells using PAX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
