Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane1: 20ug RH30 lysate, Lane2: 20ug RH30 lysate, Lane3: 20ug RH41 lysate, Lane4: 20ug RH41 lysate).Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Product Name | PAX3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PAX3 is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t (2;13) (q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma.This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t (2;13) (q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t (2;13) (q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini.1) Mercado, G.E., (2008) Genes Chromosomes Cancer 47 (6), 510-520. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAX3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PAX3 antibody can be used for detection of PAX3 by ELISA at 1:62500. PAX3 antibody can be used for detection of PAX3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAX3 |
|---|---|
| Alternative Names | PAX3 CDHS HUP2 MGC120381 MGC120382 MGC120383 MGC120384 MGC134778 WS1 WS3 |
| Molecular Weight(MW) | 53 kDa |
Application
Antibody used in WB on Human cell lines at: 1:1000 (Lane1: 20ug RH30 lysate, Lane2: 20ug RH30 lysate, Lane3: 20ug RH41 lysate, Lane4: 20ug RH41 lysate).
Application
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | PAX3 antibody can be used for detection of PAX3 by ELISA at 1:62500. PAX3 antibody can be used for detection of PAX3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAX3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
