Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Product Name | PBEF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PBEF1, 1110035O14Rik, DKFZP666B131, MGC117256, NAMPT, PBEF, VF, PBEF1, VISFATIN |
| Product description | PBEF1 catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.1) Chen, M.P., (2006) J. Clin. Endocrinol. Metab. 91 (1), 295-299. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PBEF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PBEF1 antibody can be used for detection of PBEF1 by ELISA at 1:312500. PBEF1 antibody can be used for detection of PBEF1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRatZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NAMPT |
|---|---|
| Alternative Names | PBEF1 1110035O14Rik DKFZP666B131 MGC117256 NAMPT PBEF VF PBEF1 VISFATIN |
| Molecular Weight(MW) | 54 kDa |
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PBEF1 antibody can be used for detection of PBEF1 by ELISA at 1:312500. PBEF1 antibody can be used for detection of PBEF1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PBEF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
