Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of in Y79 cell line lysates (35ug/lane)Application
Western blot analysis of in mouse stomach tissue lysates (35ug/lane)Application
Western blot analysis of lysates from K562, HepG2, HT-1080, rat C6 cell line and human liver tissue lysate (from left to right), using at 1:1000 at each lane.Application
Immunohistochemical analysis of paraffin-embedded H. brain section using . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Immunohistochemical analysis of paraffin-embedded H. liver section using . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Fluorescent image of HepG2 cells stained with XAF1 . Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Product Name | PDIA6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, ER protein 5, ERp5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, PDIA6, ERP5, P5, TXNDC7 |
| Product description | Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.1) Hayano,T.,Gene 164 (2), 377-378 (1995) |
| Immunogen | This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human PDIA6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For IF starting dilution is: 1:100 For IHC-P starting dilution is: 1:100 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Symbol | PDIA6 |
|---|---|
| Alternative Names | Protein disulfide-isomerase A6 Endoplasmic reticulum protein 5 ER protein 5 ERp5 Protein disulfide isomerase P5 Thioredoxin domain-containing protein 7 PDIA6 ERP5 P5 TXNDC7 |
| Molecular Weight(MW) | 48 kDa |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of in Y79 cell line lysates (35ug/lane)
Application
Western blot analysis of in mouse stomach tissue lysates (35ug/lane)
Application
Western blot analysis of lysates from K562, HepG2, HT-1080, rat C6 cell line and human liver tissue lysate (from left to right), using at 1:1000 at each lane.
Application
Immunohistochemical analysis of paraffin-embedded H. brain section using . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded H. liver section using . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Fluorescent image of HepG2 cells stained with XAF1 . Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Application Notes | For IF starting dilution is: 1:100 For IHC-P starting dilution is: 1:100 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
