Western blot
500 ug Human NT2 cell lysateAmount of IP Antibody :6 ugPrimary Antibody :PDIA6Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :PDIA6Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical ScienceImmunofluorescence
Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody Secondary Antibody: Alexa goat anti-rabbit 594 Image Submitted by: Yuzhi Chen, University of Arkansas for Medical SciencesWestern blot
Mouse Brain lysateWestern blot
Host: Rabbit Target Name: WT1 Sample Tissue: 721_B Antibody Dilution: 1.0ug/mlWestern blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: HepG2 Antibody Dilution: 1.0ug/mlWestern blot
Sample Type: 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical ScienceWestern blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysate| Product Name | Rabbit anti-PDIA6 polyclonal antibody - N-terminal region |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ERP5, P5, TXNDC7 |
| Immunogen | The immunogen for anti-PDIA6 antibody: synthetic peptide directed towards the N terminal of human PDIA6 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | BovineDogHorseHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDIA6 |
|---|---|
| Gene Synonyms | ERP5 P5 TXNDC7 |
| Gene Full Name | Protein disulfide isomerase family A, member 6 |
| Gene Summary | Protein disulfide isomerases, such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins. |
| Alternative Names | ERP5 P5 TXNDC7 |
| Molecular Weight(MW) | 48kDa |
| Sequence | 440 amino acids |
Western blot
500 ug Human NT2 cell lysateAmount of IP Antibody :6 ugPrimary Antibody :PDIA6Primary Antibody Dilution :1:500Secondary Antibody :Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution :1:5000Gene Name :PDIA6Submitted by :Dr. Yuzhi Chen, University of Arkansas for Medical Science
Immunofluorescence
Sample Type: NT2 cells Red: Antibody Blue: DAPI Primary Dilution: 1ug/50ul antibody Secondary Antibody: Alexa goat anti-rabbit 594 Image Submitted by: Yuzhi Chen, University of Arkansas for Medical Sciences
Western blot
Mouse Brain lysate
Western blot
Host: Rabbit Target Name: WT1 Sample Tissue: 721_B Antibody Dilution: 1.0ug/ml
Western blot
Host: Rabbit Target Name: EGFL8 Sample Tissue: HepG2 Antibody Dilution: 1.0ug/ml
Western blot
Sample Type: 2. mouse brain extracts (80ug) Primary Antibody Dilution: 2ug/ml Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience Secondary Antibody Dilution: 1: 20,000 Image Submitted by: Yuzhi Chen University of Arkansas for Medical Science
Western blot
0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 721_B cell lysate| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
