Application
Flow cytometric analysis of hela cells using (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of in NCI-H460, MCF-7, A2058 cell line lysates (35ug/lane)| Product Name | PDIA6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein disulfide-isomerase A6, Endoplasmic reticulum protein 5, ER protein 5, ERp5, Protein disulfide isomerase P5, Thioredoxin domain-containing protein 7, PDIA6, ERP5, P5, TXNDC7 |
| Product description | Protein disulfide isomerases (EC 5.3.4.1), such as PDIA6, are endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins.1) Hayano,T.,Gene 164 (2), 377-378 (1995) |
| Immunogen | This PDIA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human PDIA6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PDIA6 |
|---|---|
| Alternative Names | Protein disulfide-isomerase A6 Endoplasmic reticulum protein 5 ER protein 5 ERp5 Protein disulfide isomerase P5 Thioredoxin domain-containing protein 7 PDIA6 ERP5 P5 TXNDC7 |
| Molecular Weight(MW) | 48 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of hela cells using (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of in NCI-H460, MCF-7, A2058 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
