Application
Antibody used in WB on Human Huh7, HepG2 at: 1:50.Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Muscle.| Product Name | PDK4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK4, FLJ40832, PDHK4 |
| Product description | PDK4 is a member of the PDK/BCKDK protein kinase family and is a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism.This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.1) Kimura, K., (2006) Genome Res. 16 (1), 55-65. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDK4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PDK4 antibody can be used for detection of PDK4 by ELISA at 1:312500. PDK4 antibody can be used for detection of PDK4 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDK4 |
|---|---|
| Alternative Names | PDK4 FLJ40832 PDHK4 |
| Molecular Weight(MW) | 46 kDa |
Application
Antibody used in WB on Human Huh7, HepG2 at: 1:50.
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Muscle.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | PDK4 antibody can be used for detection of PDK4 by ELISA at 1:312500. PDK4 antibody can be used for detection of PDK4 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PDK4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
