Application
Western blot analysis of extracts from Jurkat tissue using PECAM-1 (Phospho-Tyr713) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | PECAM-1 (phospho Tyr713) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD31, CD31 antigen, EndoCAM, PEC1, PECAM |
| Product description | Induces susceptibility to atherosclerosis By similarity. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte.1) Garnacho C, et al. (2008) Blood 111, 3024-33. Udell CM, et al. (2006) J Biol Chem 281, 20949-57 . O'Brien CD, Cao G, Makrigiannakis A, DeLisser HM (2004)Am J Physiol Cell Physiol 287, C1103-13. |
| Immunogen | PECAM-1 (phospho Tyr713) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine 713 (T-V-Y (p)-S-E) derived from Human PECAM-1. |
| Modification | p-Tyr713 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PECAM1 |
|---|---|
| Alternative Names | CD31 CD31 antigen EndoCAM PEC1 PECAM |
| Molecular Weight(MW) | 82, 150 kDa |
| Tissue Specificity | The antibody detects endogenous level of PECAM-1 only when phosphorylated at tyrosine 713. |
| Entrez Gene | 5175 |
|---|---|
| Protein Accession | NP_000433.4 |
Application
Western blot analysis of extracts from Jurkat tissue using PECAM-1 (Phospho-Tyr713) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
