IHC
CD31&Emcn staining of histological sections from different groups. Green fluorescence: CD31; Red fluorescence: Emcn; Blue fluorescence: nucleus of the cells; Quantification of CD31&Emcn double positive staining of different groups, N≥ 8, p< 0.05 (Independent t-test).IF
(B) Immunofluorescence staining: Green fluorescence indicates active CD31; red fluorescence indicates active Emcn, TRAP and OCN respectively; blue fluorescence indicates PI bound to the nuclei of cells.| Product Name | PECAM1 / CD31 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide from human PECAM-1. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIF-PIHCWB |
| PECAM1 / CD31 Antibody can be used for ELISA starting at 1:20000, Immunofluorescence starting at 1:100 - 1:500, Immunohistochemistry in paraffin embeded tissues starting at 10μg/mL, and Western Blotting starting at 1:500 - 1:1000.: |
|
| Species Reactivity | HumanMouseRhesus Monkey |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PECAM1 |
|---|---|
| Alternative Names | PECAM1 CD31 CD31 antigen EndoCAM PECA1 PECAM-1 GPIIA |
| Tissue Specificity | PECAM-1 Antibody detects endogenous levels of total PECAM-1 protein. |
IHC
CD31&Emcn staining of histological sections from different groups. Green fluorescence: CD31; Red fluorescence: Emcn; Blue fluorescence: nucleus of the cells; Quantification of CD31&Emcn double positive staining of different groups, N≥ 8, p< 0.05 (Independent t-test).
IF
(B) Immunofluorescence staining: Green fluorescence indicates active CD31; red fluorescence indicates active Emcn, TRAP and OCN respectively; blue fluorescence indicates PI bound to the nuclei of cells.| Application Notes | PECAM1 / CD31 Antibody can be used for ELISA starting at 1:20000, Immunofluorescence starting at 1:100 - 1:500, Immunohistochemistry in paraffin embeded tissues starting at 10μg/mL, and Western Blotting starting at 1:500 - 1:1000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Antibody may be stored short term at 4˚C, long term aliquot and store at -20˚C, avoid freeze thaw cycles. |
| Storage Buffer | PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol |
