Application
The immunogen is recombinant mouse perforin, amino acids 98-534. Anti-Perforin (MAb CE2.10) recognizes an epitope within amino acids 402-534. Schematic structure of mouse perforin (recombinant, aa. 98-534) as seen in this image.| Product Name | Perforin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PFP, Perforin-1, Cytolysin, P1, PFP, FLH2, PFN1, HPLH2 |
| Product description | 1) Perforin and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade: P. Li, et al.; Cell 91, 479 (1997). |
| Immunogen | Perforin (monoclonal CE2.10) antibody was raised against recombinant mouse perforin (aa. 98-534). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | IPWB |
| Perforin antibody can be used for detection of horse Perforin by western blot. This product also recognizes human, mouse, rat, sheep, goat and monkey apoPerforin. Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRF1 |
|---|---|
| Alternative Names | PFP Perforin-1 Cytolysin P1 PFP FLH2 PFN1 HPLH2 |
Application
The immunogen is recombinant mouse perforin, amino acids 98-534. Anti-Perforin (MAb CE2.10) recognizes an epitope within amino acids 402-534. Schematic structure of mouse perforin (recombinant, aa. 98-534) as seen in this image.| Application Notes | Perforin antibody can be used for detection of horse Perforin by western blot. This product also recognizes human, mouse, rat, sheep, goat and monkey apoPerforin. Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CE2.10 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Perforin antibody can be stored at 4˚C, stable for one year. |
| Storage Buffer | PBS containing 0.02% sodium azide |
